Little Mississippi River Conservation Reserve is a Conservation area located in Mayo, Ontario.

• Latitude: 45° 7' 8'' North   (decimal: 45.118991)
• Longitude: 77° 33' 38'' West   (decimal: -77.560574)
• Topography Feature Category: Conservation area
• Geographical Feature: Conservation Reserve
• Canadian Province/Territory: Ontario
• Location: Mayo
• Atlas of Canada Locator Map: Little Mississippi River Conservation Reserve
• GPS Coordinate Locator Map: Little Mississippi River Conservation Reserve Lat/Long